abstract：:Salmonella serovars were examined for the presence of fim gene sequences using specific DNA probes. All strains, regardless of their ability to express surface-associated fimbriae, retain a considerable amount of DNA homologous to the gene probes used. The phenotypically nonfimbriae FIRN and non-FIRN strains of S. typ...

journal_title：Microbial pathogenesis

authors： Swenson DL,Clegg S,Old DC

更新日期：1991-06-01 00:00:00

abstract：:Outer membrane and secreted proteins were isolated from Leptospira interrogans serovar pomona and characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoblot and radioimmunoprecipitation techniques. The L. interrogans outer membranes were extracted with Triton X-114 and contained several pro...

journal_title：Microbial pathogenesis

authors： Zuerner RL,Knudtson W,Bolin CA,Trueba G

更新日期：1991-04-01 00:00:00

abstract：:The live oral typhoid vaccine Ty21a has proved to confer protection against the disease at least as effectively as killed parenteral vaccines, whereas killed oral vaccines have not been protective in field trials. This prompted us to compare the immune response of subjects vaccinated either with live oral, killed oral...

journal_title：Microbial pathogenesis

authors： Kantele A,Arvilommi H,Kantele JM,Rintala L,Mäkelä PH

更新日期：1991-02-01 00:00:00

abstract：:A number of different techniques were used to analyse classic and atypical serogroup O:11 Aeromonas isolates. Five of seven atypical O:11, S layer-negative strains lacking a homogeneous LPS side-chain pattern exhibited varying degrees of mouse pathogenecity. One virulent atypical strain (AH-77) synthesized a surface a...

journal_title：Microbial pathogenesis

authors： Kokka RP,Vedros NA,Janda JM

更新日期：1991-01-01 00:00:00

abstract：:The antigenic site normally situated in the EF loop of VP2 (2EF) of poliovirus type 2 (Lansing) [PV2 (L)] was expressed in 2EF or in the BC loop of VP1 (1BC) of PV1 (Mahoney) [PV1 (M)]. A hybrid virus expressing the site in 2EF of PV1 (M) is known to be neutralizable by PV2 (L)-specific antisera and to induce neutrali...

journal_title：Microbial pathogenesis

authors： Murdin AD,Mirzayan C,Kameda A,Wimmer E

更新日期：1991-01-01 00:00:00

abstract：:Iron is an environmental signal which regulates the coordinate expression of genes associated with virulence in many pathogenic bacteria. In response to iron-deprivation, lysogenic Corynebacterium diphtheriae C7 (beta) synthesizes and secretes diphtheria toxin and siderophore and induces a high-affinity iron uptake sy...

journal_title：Microbial pathogenesis

authors： Tai SP,Krafft AE,Nootheti P,Holmes RK

更新日期：1990-10-01 00:00:00

abstract：:Opa-expressing variants of Neisseria gonorrhoeae strain F62-SF and an Opa- variant, all non-piliated, were examined for differences in the interaction of the bacteria within colonies and in attachment to and damage of human fallopian tube mucosa. Expression of certain Opas was associated with the formation of transpar...

journal_title：Microbial pathogenesis

authors： Dekker NP,Lammel CJ,Mandrell RE,Brooks GF

更新日期：1990-07-01 00:00:00

abstract：:This paper describes the development of a murine bank of monoclonal antibodies against Bordetella pertussis toxin, filamentous hemagglutinin (FHA), pili, lipopolysaccharide (LPS), or outer membrane proteins (OMPs). Subunits S1, S2, S3 of pertussis toxin (PT) bound immunoglobulins and glycoproteins such as fetuin and h...

journal_title：Microbial pathogenesis

authors： Poolman JT,Kuipers B,Vogel ML,Hamstra HJ,Nagel J

更新日期：1990-06-01 00:00:00

abstract：:A trans-acting, positive effector of invasion plasmid antigen (Ipa) synthesis has been identified and mapped on the pWR100 invasion plasmid of Shigella flexneri serotype 5 (strain M90T-W). Recombinant plasmids carrying this regulatory gene, designated ipaR, were found to restore full virulence to a non-invasive ipaR::...

journal_title：Microbial pathogenesis

authors： Buysse JM,Venkatesan M,Mills JA,Oaks EV

更新日期：1990-03-01 00:00:00

abstract：:Fimbrial adhesins and hemolysins contribute to pathogenicity of extraintestinal Escherichia coli isolates causing urinary tract infections (UTI), sepsis and new born meningitis (NBM). Using gene cloning techniques and pulse field electrophoresis in combination with Southern hybridizations it was demonstrated that the ...

journal_title：Microbial pathogenesis

authors： Hacker J,Bender L,Ott M,Wingender J,Lund B,Marre R,Goebel W

更新日期：1990-03-01 00:00:00

abstract：:Corynebacterium pseudotuberculosis produces a sphingomyelin-specific phospholipase D exotoxin that is a major determinant in the pathogenesis of caseous lymphadenitis. We assessed the changes induced in the morphology and sphingomyelin concentration of ovine erythrocytes after incubation with broth culture filtrates o...

journal_title：Microbial pathogenesis

authors： Brogden KA,Engen RL,Songer JG,Gallagher J

更新日期：1990-02-01 00:00:00

abstract：:Influence of both recombinant interferon gamma (r-IFN-gamma) and monoclonal anti-IFN-gamma on murine Salmonella typhimurium infection was studied in vivo. As a challenge we used either a virulent or an avirulent strain of S. typhimurium. The avirulent strain is unable to replicate in the mouse spleen. The effect of IF...

journal_title：Microbial pathogenesis

authors： Muotiala A,Mäkelä PH

更新日期：1990-02-01 00:00:00

abstract：:T lymphocyte-mediated immunity is important for resistance to Francisella tularensis. To characterize the specificity of this immunity, we used membrane proteins and two lipopolysaccharide (LPS) preparations. Both membrane proteins were heat-modifiable, as indicated by their migration in sodium dodecyl sulphate polyac...

journal_title：Microbial pathogenesis

authors： Surcel HM,Sarvas M,Helander IM,Herva E

更新日期：1989-12-01 00:00:00

abstract：:The enteroinvasive species Shigella flexneri expresses a plasmid-mediated capacity to penetrate epithelial cells by directed phagocytosis involving actin polymerization. In the present work, HEp2 cells were depleted of intracellular K+ in order to arrest receptor-mediated endocytosis and to evaluate the role of this e...

journal_title：Microbial pathogenesis

authors： Clerc PL,Sansonetti PJ

更新日期：1989-11-01 00:00:00

abstract：:The reactivity of anti-pilus antibodies to native and denatured Haemophilus influenzae b (Hib) pili was studied using rabbit serum prepared against piliated H. influenzae b strain M43 (p+) and adsorbed with its non-piliated variant, strain M42 (p-). The specificity of the adsorbed serum for Hib pili was documented by ...

journal_title：Microbial pathogenesis

authors： Gilsdorf JR,Forney LJ,LiPuma JJ

更新日期：1989-10-01 00:00:00

abstract：:In an investigation of the role of monokines in CMV associated immunosuppression we document modulation of both TNF and IL-1 beta in CMV infected THP-1 cells. CMV infected cultures released almost two-fold more IL-1 beta protein and contained significantly higher IL-1 beta mRNA levels than uninfected cultures for 72-9...

journal_title：Microbial pathogenesis

authors： Turtinen LW,Assimacopoulos A,Haase AT

更新日期：1989-08-01 00:00:00

abstract：:The cAMP content of Trypanosoma brucei increases in parallel with ascending mammalian parasitemia to very high levels just before differentiation of the long-slender to the short-stumpy bloodstream form. Because expression of myc oncogenes is required for vertebrate cells to interpret proliferation signals and decline...

journal_title：Microbial pathogenesis

authors： Davis CE,Colmerauer ME,Kim CH,Matthews B,Guiney DG

更新日期：1989-07-01 00:00:00

abstract：:The addition of maltose to the growth media of Actinobacillus pleuropneumonia (serotype 1) resulted in the induction of an outer membrane protein (OMP) with a molecular mass of 42 kDa. This protein had porin-like properties in that it was peptidoglycan-associated and was resistant to proteolysis by trypsin. A pleuropn...

journal_title：Microbial pathogenesis

authors： Deneer HG,Potter AA

更新日期：1989-06-01 00:00:00

abstract：:The non-fimbrial adhesin NFA-4 from uropathogenic Escherichia coli O7:K98:H6 mediates the agglutination of human red cells (RBC), notably of blood group MM. The adhesin can be separated from the bacteria by heat extraction and was purified to homogeneity by ammonium sulphate precipitation and anion exchange chromatogr...

journal_title：Microbial pathogenesis

authors： Hoschützky H,Nimmich W,Lottspeich F,Jann K

更新日期：1989-05-01 00:00:00

abstract：:A specific mistranslation mechanism for the replication of an infectious protein is described. The feedback mechanism requires the infectious agent to induce concerted frameshifts during the translation of a cellular gene. Each module of the tandem repeat region of the gene encoding the prion protein (PrP) associated ...

journal_title：Microbial pathogenesis

authors： Wills PR

更新日期：1989-04-01 00:00:00

abstract：:Pathogenic yersiniae undergo an established low calcium response (LCR) at 37 degrees C in Ca2+-deficient media characterized by restricted growth with synthesis of Lcr plasmid-encoded virulence functions. The latter include outer membrane peptides (Yops) known to undergo Pst plasmid-mediated post-translational degrada...

journal_title：Microbial pathogenesis

authors： Mehigh RJ,Sample AK,Brubaker RR

更新日期：1989-03-01 00:00:00

abstract：:Protective immunity against Salmonella infection was studied in a mouse model. To study specificity of protection we used smooth (O-4,5,12) and rough vaccines; live and killed vaccines of both types were compared. The protection was assessed by enumerating the number of bacteria in the livers, and by following surviva...

journal_title：Microbial pathogenesis

authors： Muotiala A,Hovi M,Mäkelä PH

更新日期：1989-01-01 00:00:00

abstract：:We have developed a system whereby heterologous DNA encoding an antigen from an enteropathogen may be recombined into the chromosome of an attenuated Salmonella carrier strain. The system involves two steps: (i) integration of a hisOG deletion mutation into the chromosome; (ii) replacement of the hisOG deletion by the...

journal_title：Microbial pathogenesis

authors： Hone D,Attridge S,van den Bosch L,Hackett J

更新日期：1988-12-01 00:00:00

abstract：:We report the development of an in vitro assay using HEp-2 cell monolayers in which cell association of five C. jejuni strains and E. coli K12 C600 were tested under different experimental conditions. Both total cell associated colony forming units (CFU) and the CFU protected from gentamicin killing (i.e. internalized...

journal_title：Microbial pathogenesis

authors： de Melo MA,Pechère JC

更新日期：1988-07-01 00:00:00

abstract：:Streptococcus pneumoniae attach to human pharyngeal epithelial cells through the specific interaction of bacterial surface adhesins with glycoconjugate receptors. The present study defines the adhesin as a molecule bridging between an anchoring site in the bacterial cell wall and the epithelial cell receptor. The natu...

journal_title：Microbial pathogenesis

authors： Andersson B,Beachey EH,Tomasz A,Tuomanen E,Svanborg-Edén C

更新日期：1988-04-01 00:00:00

abstract：:We previously demonstrated that incubation of E. coli in normal human serum (NHS) resulted in the release of a finite fraction (approximately 30%) of LPS from the bacterial outer membrane. In experiments reported here, we examined factors which may enhance or diminish the capacity of NHS to mediate this limited LPS re...

journal_title：Microbial pathogenesis

authors： Tesh VL,Morrison DC

更新日期：1988-03-01 00:00:00

abstract：:Meningococci can be classified by the presence of serotype and subtype antigens on the class 2/3 and class 1 outer membrane proteins, respectively. A typing system employing monoclonal antibodies and a WCE for detection of such antigens enabled us to sero- and/or subtype 255 out of 268 group B meningococci from 19 cou...

journal_title：Microbial pathogenesis

authors： Abdillahi H,Poolman JT

更新日期：1988-01-01 00:00:00

abstract：:Pertussis toxin (PT) is an important virulence determinant of Bordetella pertussis and one of the major protective antigens against whooping cough. The genes coding for PT have recently been cloned, but attempts to express them in Escherichia coli have been unsuccessful. We therefore explored the possibility of expres...

journal_title：Microbial pathogenesis

authors： Runeberg-Nyman K,Engström O,Löfdahl S,Ylöstalo S,Sarvas M

更新日期：1987-12-01 00:00:00

abstract：:Group G streptococci have on their surface antiphagocytic M protein-like antigens. To determine if these organisms have genes similar to the M protein genes of Streptococcus pyogenes (group A), DNA from independent group G isolates of human and animal origin were tested for homology to probes representing sequences en...

journal_title：Microbial pathogenesis

authors： Simpson WJ,Robbins JC,Cleary PP

更新日期：1987-11-01 00:00:00

abstract：:The accumulation of inflammatory cells in the peritoneal cavity of C57BL/6 mice was examined following intraperitoneal injection of Entamoeba histolytica trophozoites. Two different strains of E. histolytica were used: a virulent strain (IP:0682:1) and a non-virulent strain (DKB). Injection of 10(6) trophozoites of ei...

journal_title：Microbial pathogenesis

authors： Ghadirian E,Stevenson MM

更新日期：1987-09-01 00:00:00

abstract：:A cytotoxin to Vero cells (Shiga-like toxin), which was neutralized by antibody against purified Shiga toxin produced by Shigella dysenteriae 1, was purified from Escherichia coli O157:H7, isolated from a patient with hemorrhagic colitis. The purification procedure consisted of ammonium sulfate fractionation, DEAE-cel...

journal_title：Microbial pathogenesis

authors： Noda M,Yutsudo T,Nakabayashi N,Hirayama T,Takeda Y

更新日期：1987-05-01 00:00:00

abstract：:Avian sarcoma virus (ASV)-induced tumor cells that are derived from regressing neoplasms contain about 75% less pp60src kinase activity than do progressively-growing tumor cells. Paradoxically, the polyadenylated RNAs extracted from 'regressor' cells found in regressing neoplasms are much more productive than those fr...

journal_title：Microbial pathogenesis

authors： Poulin L,Wainberg MA

更新日期：1987-02-01 00:00:00

abstract：:The gene coding for serotype A of epidermolytic (exfoliative) toxin has been cloned from Staphylococcus aureus in Escherichia coli phage lambda and plasmid vectors. The coding sequence for eta was localised by subcloning and transposon Tn5 mutagenesis experiments. The eta gene was probably expressed from its natural p...

journal_title：Microbial pathogenesis

authors： O'Toole PW,Foster TJ

更新日期：1986-12-01 00:00:00

abstract：:Enteropathogenic strains of Yersinia enterocolitica harbor a virulence plasmid (pYVe plasmid) of 70 kilobases (kb) which specifies, at 37 degrees C, a calcium requirement for growth, autoagglutinability, resistance to the bactericidal activity of human serum, and the expression of outer membrane proteins (OMP). Some m...

journal_title：Microbial pathogenesis

authors： Cornelis G,Sory MP,Laroche Y,Derclaye I

更新日期：1986-08-01 00:00:00

abstract：:A virus inhibitor found in gastric secretions and in extracts from gastrointestinal tissues is described. The inhibitor shares a number of characteristics with the recently described contact-blocking virus inhibitor (CVI), which is produced by unstimulated cells in culture, and occurs naturally in some body fluids. Th...

journal_title：Microbial pathogenesis

authors： Baron JL,Li JL,McKerlie ML,Shabot JM,Coppenhaver DH

更新日期：1986-06-01 00:00:00

abstract：:A phage lambda mediated transduction system was devised to facilitate molecular analysis of Vibrio cholerae. A lamB expression plasmid, pAMH62 was introduced into Vibrio cholerae by conjugation. The resulting V. cholerae derivatives harboring pAMH62 produced substantial amounts of the LamB protein. This protein was pr...

journal_title：Microbial pathogenesis

authors： Harkki A,Hirst TR,Holmgren J,Palva ET

更新日期：1986-06-01 00:00:00

abstract：:High-affinity binding sites for P-fimbriated and for 075X-positive Escherichia coli were located in the human kidney. Frozen sections of normal human kidney were double-stained first with fluorochrome-labelled bacteria and then with fluorochrome-labelled nephron site-specific lectins or antibodies. The P-fimbriate rec...

journal_title：Microbial pathogenesis

authors： Nowicki B,Holthöfer H,Saraneva T,Rhen M,Väisänen-Rhen V,Korhonen TK

更新日期：1986-04-01 00:00:00

abstract：:The gene clusters encoding various P-fimbriae (F7(1), F7(2), F9 and F11) were compared. Deletion plasmids that lack the gene encoding the fimbrillin were derived from these gene clusters. Introduction of these deletion plasmids into an E. coli K12 strain resulted in non-fimbriated cells that still showed mannose-resis...

journal_title：Microbial pathogenesis

authors： van Die I,Zuidweg E,Hoekstra W,Bergmans H

更新日期：1986-02-01 00:00:00